Yes, negative marking applies to certain question types in the GATE exam. For Multiple Choice Questions (MCQs), a deduction of one-third mark is made for each incorrect answer in 1-mark questions and two-thirds mark for each incorrect answer in 2-mark questions. However, there is no negative marking for Multiple Select Questions (MSQs) and Numerical Answer Type (NAT) questions. Candidates should carefully read each question type and attempt answers strategically to minimize loss of marks due to incorrect responses.